ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease T1 | ||
| Ligand name |
Cytosine-2'-monophosphate
 Cytosine-2'-monophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.0 | ± | |
| ΔG | -17.24 | ± | kJ/mol |
| ΔH | -31.42 | ± 0.96 | kJ/mol |
| -TΔS | -14.18 | ± | kJ/mol |
| KD | 578.03 | ± 7692.31 | uM |
| ΔCP | -0.15 | ± 0.0 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard errors of the mean. |
General comments |
Number of titrations: 3. n was held equal to 1 during the curve-fitting process. |
| Warnings |
Affinity (578.0347) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-17.24) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 278.15 | pH | 5.5 |
| Buffer | Sodium Acetate 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Phys. Chem. [1992] 96, 4052-4056 | ||