ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Streptavidin (synthetic construct) | ||
| Ligand name |
HABA
 HABA |
||
| Interaction type | Misc | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.12 | ± 0.07 | |
| ΔG | -22.05 | ± 2.09 | kJ/mol |
| ΔH | 7.11 | ± 0.42 | kJ/mol |
| -TΔS | 29.16 | ± 1.67 | kJ/mol |
| KD | 137.08 | ± 1805.05 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Date for average of three experiments, error parameters represent +/- 1 standard deviation. |
General comments | |
| Warnings |
DeltaH (7.11) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 6.9 |
| Buffer | Potassium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [1992] 114, 3197-3200 | ||