ITC

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ITC datum

Protein and Ligand
Protein name Streptavidin (synthetic construct)
Ligand name Biotin Biotin diagram
Biotin
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 0.86 ±  
ΔG -76.57 ± kJ/mol
ΔH -133.89 ± kJ/mol
-TΔS -57.32 ± kJ/mol
KD 0.0 ± uM
ΔCP ±
Notes on error determination Not applicable
General comments Only ΔH and n were fitting parameters for the obsereved reaction heat for d-biotin with Ka fixed at the litterature value of 2.5E13.
Warnings Affinity (0.0000) less than 0.0000. It is difficult to interpred ITC data if Affinity is less than 0.0000 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
DeltaG (-76.57) less than -60.00. It is difficult to interpred ITC data if DeltaG is less than -60.00 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
Instrument Omega ITC Microcalorimeter
Temp. (K) 298.15 pH 6.9
Buffer Potassium Chloride 100.00 mM
Cell content Protein
Reference J. Am. Chem. Soc. [1992] 114, 3197-3200