ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Streptavidin (synthetic construct) | ||
| Ligand name |
3'-methyl-HABA
 3'-methyl-HABA |
||
| Interaction type | Misc | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.13 | ± 0.14 | |
| ΔG | -30.25 | ± | kJ/mol |
| ΔH | 5.36 | ± | kJ/mol |
| -TΔS | 35.61 | ± | kJ/mol |
| KD | 5.0 | ± 33333.33 | uM |
| ΔCP | ± | ||
| Notes on error determination |
The reported values are the mean for repeated experiments and errors are +/- 1 SD. |
General comments | |
| Warnings |
DeltaH (5.36) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 6.9 |
| Buffer | Potassium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [1994] 116, 2717-2724 | ||