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ITC datum

Protein and Ligand
Protein name Trypsin
Ligand name N-alpha-(2-naphthylsulfonyl)-n-(3-amidino-l-phenylalaninyl)-d-pipecolinic acid-variant-1bme N-alpha-(2-naphthylsulfonyl)-n-(3-amidino-l-phenylalaninyl)-d-pipecolinic acid-variant-1bme diagram
N-alpha-(2-naphthylsulfonyl)-n-(3-amidino-l-phenylalaninyl)-d-pipecolinic acid-variant-1bme
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -37.0 ± kJ/mol
ΔH -16.9 ± 0.8 kJ/mol
-TΔS 20.6 ± -1.2 kJ/mol
KD 0.32 ± 0.91 uM
ΔCP -2.79 ± 0.11 kJ/mol/K
Notes on error determination Not available
General comments
Warnings Table 3 (row 3) ambigous typo/error: ΔG != ΔH - TΔS -37 != -16.9 + -20.6 Difference: 0.5 Authors contacted.
DeltaG (-37.00) != DeltaH (-16.90) - T.DeltaS (20.60) (=-37.50). Tolerance range from -37.37 to -36.63
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.8
Buffer Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2001] 313, 593-614