ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | c-Src SH2 Domain (C188A) | ||
| Ligand name |
Compound 13, charifson et al.
 Compound 13, charifson et al. |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -35.56 | ± | kJ/mol |
| ΔH | -7.53 | ± 1.26 | kJ/mol |
| -TΔS | 27.82 | ± | kJ/mol |
| KD | 0.6 | ± 0.1 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not available |
General comments | |
| Warnings |
Rounding errors DeltaH (-7.53) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 8.0 |
| Buffer | HEPES 20.00 mM Sodium Chloride 150.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1997] 36, 6283-6293 | ||