ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | HIV-1 Protease (I13V/E35D/M36I/S37N/R41K/R57K/H69K/V82F/I84V/L89M) | ||
| Ligand name |
Ritonavir
 Ritonavir |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -40.58 | ± | kJ/mol |
| ΔH | -5.02 | ± | kJ/mol |
| -TΔS | 35.56 | ± | kJ/mol |
| KD | 0.08 | ± 0.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not available |
General comments |
Displacement titration experiments. Concentration of acetylpepstatin in the titration cell was 200 micromolar. |
| Warnings |
DeltaH (-5.02) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 5.0 |
| Buffer | DMSO 2.00 % Sodium Acetate 10.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2002] 41, 8613-8619 | ||