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Structure-Calorimetry Of Reported Protein Interactions Online

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ITC

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ITC datum

Protein and Ligand
Protein name	Trypsin
Ligand name	N-alpha-(2-naphthylsulfonyl)-3-amidino-l-phenylalanine piperazide-version-2 N-alpha-(2-naphthylsulfonyl)-3-amidino-l-phenylalanine piperazide-version-2
Interaction type	Synthetic ligands
ITC data	ITC data
Structure data	Structure data
ITC Information
n	0.93	± 0.12
ΔG	-32.0	± 0.5	kJ/mol
ΔH	-4.4	± 3.6	kJ/mol
-TΔS	27.6	± -3.6	kJ/mol
KD	2.5	± 14.29	uM
ΔCP	-0.91	± 0.19	kJ/mol/K
Notes on error determination	Not available
General comments	Altered change in enthalpy and entropy from further studies on protonation events during binding, see http://scorpio2-dev.biophysics.ismb.lon.ac.uk/citation/view/94.
Warnings	DeltaH (-4.40) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0.
Instrument	Microcal MCS Titration Calorimeter
Temp. (K)	298.15	pH	7.8
Buffer	Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content	Protein
Reference	J Mol Biol. [2001] 313, 593-614

Disclaimer

This is an ongoing project and, although SCORPIO has been curated with great care, it may contain errors. If you spot an error please contact us. We take no responsibility for the data within SCORPIO, nor any responsibility for the consequences of using the data within SCORPIO. No contract, warranty or liability is implied by using the data within SCORPIO.

If you make use of SCORPIO please cite J. Mol. Biol. [2008] 384, 1002-1017
Tjelvar S. G. Olsson, Mark A. Williams, Will R. Pitt and John E. Ladbury