ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Alpha-Thrombin | ||
| Ligand name |
N-alpha-(2-naphthylsulfonyl)-3-amidino-l-phenylalanine piperazide-version-2et
 N-alpha-(2-naphthylsulfonyl)-3-amidino-l-phenylalanine piperazide-version-2et |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -40.3 | ± | kJ/mol |
| ΔH | -24.4 | ± 0.9 | kJ/mol |
| -TΔS | 15.9 | ± | kJ/mol |
| KD | 0.09 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not available |
General comments | |
| Warnings | |||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.8 |
| Buffer | Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2001] 313, 593-614 | ||