ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Disabled Homolog 1 (native) | ||
Ligand name |
TKSMNFDNPVYRKT
TKSMNFDNPVYRKT |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | ± | ||
ΔG | -32.22 | ± | kJ/mol |
ΔH | -40.17 | ± | kJ/mol |
-TΔS | -7.95 | ± | kJ/mol |
KD | 3.0 | ± 0.7 | uM |
ΔCP | ± | ||
Notes on error determination |
The estimated error in Kd upon repeated measurements approximately 25% (standard error of the mean) |
General comments | |
Warnings |
DeltaG (-32.22) != -R.T.ln(Ka) (-31.52). Tolerance range from -32.54 to -31.89 |
||
Instrument | Not stated | ||
Temp. (K) | 298.15 | pH | 7.0 |
Buffer | Sodium Chloride 150.00 mM PIPES 20.00 mM DTT 0.20 mM | ||
Cell content | Protein | ||
Reference | Biochemistry [2004] 43, 10979-10987 |