ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease A | ||
| Ligand name |
3'-uridinemonophosphate
 3'-uridinemonophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.93 | ± | |
| ΔG | -29.29 | ± | kJ/mol |
| ΔH | -57.74 | ± | kJ/mol |
| -TΔS | -28.45 | ± | kJ/mol |
| KD | 9.7 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-29.29) != -R.T.ln(Ka) (-28.62). Tolerance range from -29.58 to -29.00 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 6.0 |
| Buffer | Sodium Chloride 0.01 M MES - Sodium Hydroxide 0.02 M | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1998] 37, 17386-17401 | ||