ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease A | ||
| Ligand name |
3'-uridinemonophosphate
 3'-uridinemonophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.95 | ± | |
| ΔG | -25.1 | ± | kJ/mol |
| ΔH | -49.37 | ± | kJ/mol |
| -TΔS | -24.27 | ± | kJ/mol |
| KD | 54.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-25.10) != -R.T.ln(Ka) (-24.36). Tolerance range from -25.36 to -24.85 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 6.0 |
| Buffer | MES - Sodium Hydroxide 0.10 M Sodium Chloride 0.10 M | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1998] 37, 17386-17401 | ||