ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease A (K7A/R10A/K66A) | ||
| Ligand name |
3'-uridinemonophosphate
 3'-uridinemonophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.0 | ± | |
| ΔG | -25.52 | ± | kJ/mol |
| ΔH | -42.26 | ± | kJ/mol |
| -TΔS | -17.15 | ± | kJ/mol |
| KD | 47.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Table 4 (row 3) ambigous typo/error:
-25.5224 != -42.2584 + 17.1544
-0.418399999999998
Authors contacted. DeltaG (-25.52) != -R.T.ln(Ka) (-24.70). Tolerance range from -25.78 to -25.27 DeltaG (-25.52) != DeltaH (-42.26) - T.DeltaS (-17.15) (=-25.10). Tolerance range from -25.78 to -25.27 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 6.0 |
| Buffer | Sodium Chloride 0.01 M MES - Sodium Hydroxide 0.02 M | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1998] 37, 17386-17401 | ||