ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | v-Src SH2 Domain (variant 1) | ||
| Ligand name |
pYEEI
 pYEEI |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -37.7 | ± 0.04 | kJ/mol |
| ΔH | -25.36 | ± 0.21 | kJ/mol |
| -TΔS | 12.35 | ± 0.25 | kJ/mol |
| KD | 0.24 | ± 10.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Uncertainties represent standard deviation from regression analysis. |
General comments | |
| Warnings | |||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.3 |
| Buffer | HEPES 20.00 mM Beta-mercaptoethanol 1.00 mM EDTA 1.00 mM Sodium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2002] 124, 205-215 | ||