ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | v-Src SH2 Domain (variant 1) | ||
| Ligand name |
ptc-EEI
 ptc-EEI |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -39.96 | ± 0.29 | kJ/mol |
| ΔH | -18.74 | ± 0.17 | kJ/mol |
| -TΔS | 21.21 | ± 1.25 | kJ/mol |
| KD | 0.1 | ± 1.0 | uM |
| ΔCP | -0.94 | ± 0.04 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard deviation from regression analysis. |
General comments | |
| Warnings |
Corrected typo in original paper: table 2, row 2, col 3 changed to -4.48 from -5.91. Deduced from ΔG, ΔS, the temperature and the plot in figure 2.
Authors contacted. |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.3 |
| Buffer | HEPES 20.00 mM Beta-mercaptoethanol 1.00 mM EDTA 1.00 mM Sodium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2002] 124, 205-215 | ||