ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Beta-Glucosidase | ||
| Ligand name |
1-deoxynojirimycin
 1-deoxynojirimycin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.98 | ± 0.01 | |
| ΔG | -33.3 | ± | kJ/mol |
| ΔH | -47.24 | ± 0.04 | kJ/mol |
| -TΔS | -13.93 | ± | kJ/mol |
| KD | 4.76 | ± 100.0 | uM |
| ΔCP | -0.51 | ± 0.08 | kJ/mol/K |
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Table 1 (col 2) ambigious error/typo:
ΔG != ΔH - TΔS
-33.30464 != -47.23736 + 16.44312
Difference: -2.5104
Authors contacted. |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 326.86 | pH | 7.0 |
| Buffer | Potassium Phosphate 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2003] 125, 14313-14323 | ||