ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Beta-Glucosidase | ||
| Ligand name |
1-deoxynojirimycin
 1-deoxynojirimycin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.37 | ± 0.02 | |
| ΔG | -26.53 | ± 0.08 | kJ/mol |
| ΔH | -36.36 | ± 1.63 | kJ/mol |
| -TΔS | -9.83 | ± 1.59 | kJ/mol |
| KD | 35.71 | ± 1000.0 | uM |
| ΔCP | -0.51 | ± 0.08 | kJ/mol/K |
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-26.53) != -R.T.ln(Ka) (-26.24). Tolerance range from -26.79 to -26.26 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 308.15 | pH | 7.0 |
| Buffer | Potassium Phosphate 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2003] 125, 14313-14323 | ||