ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Beta-Glucosidase | ||
| Ligand name |
5-hydroxymethyl-3,4-dihydroxypiperidine
 5-hydroxymethyl-3,4-dihydroxypiperidine |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.07 | ± 0.04 | |
| ΔG | -46.65 | ± 0.96 | kJ/mol |
| ΔH | -43.18 | ± 0.08 | kJ/mol |
| -TΔS | 3.47 | ± | kJ/mol |
| KD | 0.03 | ± 1.18 | uM |
| ΔCP | -0.21 | ± 0.02 | kJ/mol/K |
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Corrected typo in original paper: table 1, row 4, col 5 changed to 0.83 from -0.83. Deduced from ΔG and ΔH.
Authors contacted |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 326.41 | pH | 5.8 |
| Buffer | MES 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2003] 125, 14313-14323 | ||