ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Beta-Glucosidase | ||
| Ligand name |
5-hydroxymethyl-3,4-dihydroxypiperidine
 5-hydroxymethyl-3,4-dihydroxypiperidine |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.36 | ± 0.02 | |
| ΔG | -42.26 | ± 0.42 | kJ/mol |
| ΔH | -31.0 | ± 0.08 | kJ/mol |
| -TΔS | 11.21 | ± 3.35 | kJ/mol |
| KD | 0.06 | ± 0.43 | uM |
| ΔCP | -0.21 | ± 0.02 | kJ/mol/K |
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-42.26) != -R.T.ln(Ka) (-41.33). Tolerance range from -42.68 to -41.84 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.0 |
| Buffer | Potassium Phosphate 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [2003] 125, 14313-14323 | ||