ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Hevein | ||
| Ligand name |
Chitobiose
 Chitobiose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -15.73 | ± | kJ/mol |
| ΔH | -20.67 | ± 0.25 | kJ/mol |
| -TΔS | -4.94 | ± | kJ/mol |
| KD | 1577.29 | ± 76923.08 | uM |
| ΔCP | -0.27 | ± 0.03 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard deviation from regression analysis. |
General comments | |
| Warnings |
Affinity (1577.2871) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-15.73) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 5.5 |
| Buffer | Sodium Acetate/HCl 0.10 M | ||
| Cell content | Protein | ||
| Reference | Proteins [1997] 29, 467-477 | ||