ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Adipocyte Lipid-Binding Protein | ||
Ligand name |
Arachidonic acid
Arachidonic acid |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 0.8 | ± 0.2 | |
ΔG | -30.88 | ± 0.54 | kJ/mol |
ΔH | -28.33 | ± 5.69 | kJ/mol |
-TΔS | 2.51 | ± 5.94 | kJ/mol |
KD | 4.4 | ± 1.1 | uM |
ΔCP | ± | ||
Notes on error determination |
Data based upon five independent isotherms |
General comments | |
Warnings | |||
Instrument | Omega ITC Microcalorimeter | ||
Temp. (K) | 299.15 | pH | 7.0 |
Buffer | Sodium Phosphate 50.00 mM Ethanol 0.10 % | ||
Cell content | Ligand | ||
Reference | J. Biol. Chem [1994] 269, 25339-25347 |