ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | DNA Gyrase B N-Terminal 24 Kda Fragment (variant 2) | ||
Ligand name |
Novobiocin
Novobiocin |
||
Interaction type | Synthetic ligands | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 1.3 | ± 0.1 | |
ΔG | -42.26 | ± 0.42 | kJ/mol |
ΔH | -51.04 | ± 0.42 | kJ/mol |
-TΔS | -8.79 | ± 0.84 | kJ/mol |
KD | 0.04 | ± 0.25 | uM |
ΔCP | -1.23 | ± | kJ/mol/K |
Notes on error determination |
The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small. |
General comments | |
Warnings | |||
Instrument | Microcal MCS Titration Calorimeter | ||
Temp. (K) | 300.0 | pH | 7.4 |
Buffer | Tris HCl 69.00 mM | ||
Cell content | Protein | ||
Reference | Biochemistry [1997] 36, 9663-9673 |