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ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 43 Kda Fragment
Ligand name Novobiocin Novobiocin diagram
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 1.1 ± 0.1  
ΔG -43.51 ± 0.42 kJ/mol
ΔH -53.14 ± 0.42 kJ/mol
-TΔS -9.62 ± 0.42 kJ/mol
KD 0.03 ± 0.2 uM
Notes on error determination The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small.
General comments
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 300.0 pH 7.4
Buffer Phosphate Buffer 20.00 mM
Cell content Protein
Reference Biochemistry [1997] 36, 9663-9673