| ITC comment | ITC comment defintion |
|---|
| Not applicable |
EXP_ERROR_DET_COMMENT |
| Errors were determined from duplicate experiments by error propagation. |
EXP_ERROR_DET_COMMENT |
| The estimated error in Kd upon repeated measurements approximately 25% (standard error of the mean) |
EXP_ERROR_DET_COMMENT |
| Mean value of three independent measurements +- s.e. |
EXP_ERROR_DET_COMMENT |
| Not available |
EXP_ERROR_DET_COMMENT |
| Uncertainties represent standard deviation from regression analysis. |
EXP_ERROR_DET_COMMENT |
| Data based upon five independent isotherms |
EXP_ERROR_DET_COMMENT |
| Data based upon three experiments |
EXP_ERROR_DET_COMMENT |
| The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small. |
EXP_ERROR_DET_COMMENT |
| Ka and delta H were obtainied from a non-linear regression fit to the isotherm and the error of these values were always less than 7% (n=4) and less than 4% (n=2). Where n is the assumed number of biniding sites. |
EXP_ERROR_DET_COMMENT |
| SBT binding was measured 3 times at 25C, other values are from single measurements. For most ligands, fitted errors in Kd, n, DH, DS, and DG values are less than or equal to 5, 2, 3, 5 and 5% respectively. For MT they are as high as 9, 11, 14, 30 and 11% |
EXP_ERROR_DET_COMMENT |
| Larger deviation estimates were obsereved for the halothane-HSA interaction. Because the number of sites for halothane has been reported as seven the data was refitted by fixing n = 7. |
EXP_ERROR_DET_COMMENT |
| In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. |
EXP_ERROR_DET_COMMENT |
| In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. Kd, G and -T.S represent upper limit values. |
EXP_ERROR_DET_COMMENT |
| Errors reported were propagated from the errors of Ka and H, respectively. |
EXP_ERROR_DET_COMMENT |
| Data were fitted to a model assuming one binding site. (Mean +/- S.D. n=3). |
EXP_ERROR_DET_COMMENT |
| The uncertainties reported for Kb and Hb are the standard deviations from the average value, determined from at least two different titrations. |
EXP_ERROR_DET_COMMENT |
| Errors represent standard deviations of three experiments. |
EXP_ERROR_DET_COMMENT |
| Data are reported as the mean fro two separate titrations with +/- 1 standard deviation in parentheses. |
EXP_ERROR_DET_COMMENT |
| Calorimetric parameters are reported as the mean value of at least two to three independent experiments, with the reported uncertainty typically being the standard deviation of multiple experiments. |
EXP_ERROR_DET_COMMENT |
