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ITC datum

Protein and Ligand
Protein name v-Src SH2 Domain (variant 2)
Ligand name PQpYIpYVPI PQpYIpYVPI diagram
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -36.4 ± 0.42 kJ/mol
ΔH -6.28 ± 0.84 kJ/mol
-TΔS 30.12 ± 0.84 kJ/mol
KD 0.4 ± 10.0 uM
Notes on error determination Calorimetric parameters are reported as the mean value of at least two to three independent experiments, with the reported uncertainty typically being the standard deviation of multiple experiments.
General comments
Warnings DeltaH (-6.28) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0.
Instrument VP-ITC Microcalorimeter
Temp. (K) 298.15 pH 7.5
Buffer HEPES 20.00 mM Beta-mercaptoethanol 1.00 mM EDTA 1.00 mM Sodium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2003] 328, 655-668