ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | v-Src SH2 Domain (R207A) | ||
| Ligand name |
PQpYIpYVPI
 PQpYIpYVPI |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -32.22 | ± 0.42 | kJ/mol |
| ΔH | -8.37 | ± 0.42 | kJ/mol |
| -TΔS | 23.85 | ± 0.42 | kJ/mol |
| KD | 1.82 | ± 33.33 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Calorimetric parameters are reported as the mean value of at least two to three independent experiments, with the reported uncertainty typically being the standard deviation of multiple experiments. |
General comments | |
| Warnings |
DeltaG (-32.22) != -R.T.ln(Ka) (-32.77). Tolerance range from -32.54 to -31.89 DeltaH (-8.37) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.5 |
| Buffer | HEPES 20.00 mM Beta-mercaptoethanol 1.00 mM EDTA 1.00 mM Sodium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2003] 328, 655-668 | ||