ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | c-Src SH2 Domain | ||
| Ligand name |
[acetyl]-[deoxy-difluoromethelene-phosphotyrosine]-E-[dipentylamine]
![[acetyl]-[deoxy-difluoromethelene-phosphotyrosine]-E-[dipentylamine] diagram](/ligand_diagrams/5.png) [acetyl]-[deoxy-difluoromethelene-phosphotyrosine]-E-[dipentylamine] |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -32.64 | ± | kJ/mol |
| ΔH | -21.34 | ± 0.84 | kJ/mol |
| -TΔS | 11.23 | ± | kJ/mol |
| KD | 2.4 | ± 0.7 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not available |
General comments | |
| Warnings |
Rounding errors DeltaG (-32.64) != -R.T.ln(Ka) (-32.08). Tolerance range from -32.96 to -32.31 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 8.0 |
| Buffer | HEPES 20.00 mM Sodium Chloride 150.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1997] 36, 6283-6293 | ||