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ITC datum

Protein and Ligand
Protein name Trypsin
Ligand name [N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine [N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine diagram
[N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -38.3 ± kJ/mol
ΔH -24.7 ± 1.2 kJ/mol
-TΔS 10.6 ± -1.2 kJ/mol
KD 0.19 ± 1.3 uM
ΔCP -1.2 ± 0.15 kJ/mol/K
Notes on error determination Not available
General comments
Warnings Table 3 (row 9) ambigous typo/error: -38.3 != -24.7 + -10.6 Difference: -3 Authors contacted.
DeltaG (-38.30) != DeltaH (-24.70) - T.DeltaS (10.60) (=-35.30). Tolerance range from -38.68 to -37.92
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.8
Buffer Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2001] 313, 593-614