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ITC datum

Protein and Ligand
Protein name Alpha-Thrombin
Ligand name [N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine [N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine diagram
[N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -49.2 ± kJ/mol
ΔH -48.5 ± 1.0 kJ/mol
-TΔS 0.7 ± kJ/mol
KD 0.0 ± uM
ΔCP ±
Notes on error determination Not available
General comments
Warnings DeltaG (-49.20) != -R.T.ln(Ka) (-53.09). Tolerance range from -49.69 to -48.71
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.8
Buffer Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2001] 313, 593-614