ITC

Browse


ISMB logo
Biochemical Journal logo

ITC datum

Protein and Ligand
Protein name Alpha-Thrombin
Ligand name {[(1r)-2-((2s)-2-{[(3-{[amino(imino)methyl]amino}propyl)amino]carbonyl}piperidinyl)-1-(cyclohexylmethyl)-2-oxoethyl]amino}acetic acid {[(1r)-2-((2s)-2-{[(3-{[amino(imino)methyl]amino}propyl)amino]carbonyl}piperidinyl)-1-(cyclohexylmethyl)-2-oxoethyl]amino}acetic acid diagram
{[(1r)-2-((2s)-2-{[(3-{[amino(imino)methyl]amino}propyl)amino]carbonyl}piperidinyl)-1-(cyclohexylmethyl)-2-oxoethyl]amino}acetic acid
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -47.8 ± kJ/mol
ΔH -57.8 ± 2.0 kJ/mol
-TΔS -10.0 ± kJ/mol
KD 0.0 ± uM
ΔCP ±
Notes on error determination Not available
General comments
Warnings
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.8
Buffer Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2001] 313, 593-614