ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Alpha-Thrombin | ||
| Ligand name |
[N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine
![[N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine diagram](/ligand_diagrams/22.png) [N-[n-(4-methoxy-2,3,6-trimethylphenylsulfonyl)-l-aspartyl]-d-(4-amidino-phenylalanyl)]-piperidine |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | ± | kJ/mol | |
| ΔH | ± | kJ/mol | |
| -TΔS | ± | kJ/mol | |
| KD | ± | uM | |
| ΔCP | -3.64 | ± 0.1 | kJ/mol/K |
| Notes on error determination |
Not available |
General comments | |
| Warnings |
Missing Affinity value Missing DeltaG value Missing DeltaH value Missing DeltaS value Missing Temperature value |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | pH | 7.8 | |
| Buffer | Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM Rubidium Chloride 100.00 mM PEG 8000 0.10 % | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2001] 313, 593-614 | ||