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Structure-Calorimetry Of Reported Protein Interactions Online

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ITC

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• ΔC_p

ITC datum

Protein and Ligand
Protein name	Alpha-Thrombin
Ligand name	[((1r)-2-{(2s)-2-[({4-[amino(imino)methyl]benzyl}amino)carbonyl]azetidinyl}-1-cyclohexyl-2-oxoethyl)amino]acetic acid [((1r)-2-{(2s)-2-[({4-[amino(imino)methyl]benzyl}amino)carbonyl]azetidinyl}-1-cyclohexyl-2-oxoethyl)amino]acetic acid
Interaction type	Synthetic ligands
ITC data	ITC data
Structure data	Structure data
ITC Information
n		±
ΔG		±	kJ/mol
ΔH		±	kJ/mol
-TΔS		±	kJ/mol
KD		±	uM
ΔCP	-2.28	± 0.2	kJ/mol/K
Notes on error determination	Not available
General comments
Warnings	Missing Affinity value Missing DeltaG value Missing DeltaH value Missing DeltaS value Missing Temperature value
Instrument	Microcal MCS Titration Calorimeter
Temp. (K)		pH	7.8
Buffer	Sodium Chloride 100.00 mM Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 %
Cell content	Protein
Reference	J Mol Biol. [2001] 313, 593-614

Disclaimer

This is an ongoing project and, although SCORPIO has been curated with great care, it may contain errors. If you spot an error please contact us. We take no responsibility for the data within SCORPIO, nor any responsibility for the consequences of using the data within SCORPIO. No contract, warranty or liability is implied by using the data within SCORPIO.

If you make use of SCORPIO please cite J. Mol. Biol. [2008] 384, 1002-1017
Tjelvar S. G. Olsson, Mark A. Williams, Will R. Pitt and John E. Ladbury