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ITC commentITC comment defintion
DeltaH (9.00) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. DUBIOUS_DATA
DeltaH (9.20) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. DUBIOUS_DATA
DeltaH (9.75) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. DUBIOUS_DATA
Displacement titration experiments. Concentration of acetylpepstatin in the titration cell was 200 micromolar. GENERAL_COMMENT
Entropy units reported should be J/mol/K GENERAL_COMMENT
Errors reported were propagated from the errors of Ka and H, respectively. EXP_ERROR_DET_COMMENT
Errors represent standard deviations of three experiments. EXP_ERROR_DET_COMMENT
Errors represent the standard deviations of triplicate or quadruplicate experiments or propagation of error. EXP_ERROR_DET_COMMENT
Errors were determined from duplicate experiments by error propagation. EXP_ERROR_DET_COMMENT
ITC binding curves at this high affinity become too steep for accurate fitting, and the estimation of contingent parameters isconsequently less reliable. GENERAL_COMMENT
In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. EXP_ERROR_DET_COMMENT
In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. Kd, G and -T.S represent upper limit values. EXP_ERROR_DET_COMMENT
Ka and delta H were obtainied from a non-linear regression fit to the isotherm and the error of these values were always less than 7% (n=4) and less than 4% (n=2). Where n is the assumed number of biniding sites. EXP_ERROR_DET_COMMENT
Larger deviation estimates were obsereved for the halothane-HSA interaction. Because the number of sites for halothane has been reported as seven the data was refitted by fixing n = 7. EXP_ERROR_DET_COMMENT
Mean value of three independent measurements +- s.e. EXP_ERROR_DET_COMMENT
Missing Affinity value MISSING_DATA
Missing DeltaG value MISSING_DATA
Missing DeltaH value MISSING_DATA
Missing DeltaS value MISSING_DATA
Missing Temperature value MISSING_DATA

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